I’ve got one more installment for our mini-series on the AdvanceBio Amino Acid Analysis discussion. We’ve already covered preventative tips to keep things running smoothly and troubleshooting common problems with this method, now let’s talk about how to save ourselves a few minutes of analysis time. For one or two samples, shaving a couple minutes off the run time may not make a big difference, but it’ll really add up in time and mobile phase savings the more samples you have to analyze.
Here’s the gradient profile for the amino acid separation, with time for the amino acid derivatization added at the beginning (this 5 minutes is an estimate – each autosampler model takes a slightly different amount of time):
As illustrated (and written in the “How to” Guide), it would take ~ 46 minutes to run two samples. However, during the sample derivatization, the autosampler is in bypass mode, so the initial mobile phase conditions are flowing through the column – that is, the column is undergoing additional equilibration while the sample is equilibrating. Instead of ending the method at 18 minutes, we end it at 15.9 minutes, immediately after returning to the initial mobile phase conditions, we save just over 2 minutes per injection. Sure, I’m generally not worried about another 2 minutes for a single sample, but to run calibration standards and a large sample set, that time and mobile phase adds up.
That’s it for amino acid analysis for now. Talk soon!
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